Mutation by Robin Cook Page A

just tell me?"
         "Because you wouldn't believe me," Victor said. "Be patient for a few moments more." He took a scalpel and inserted a fresh, razor-sharp blade.
         Marsha looked away as he quickly slit open each of the animals.
         "These are Aplasia," Victor said, trying to cover his own nervousness with a strictly scientific approach. "They have been used widely for nerve cell research." He picked up a scissor and began snipping quickly and deliberately.
         "There," he said. "I've removed the abdominal ganglion from each of the Aplasia."
         Marsha looked. Victor was holding a small flat dish filled with clear fluid. Within, floating on the surface of the liquid, were two minute pieces of tissue.
         "Now come over to the microscope," Victor said.
         "What about those poor creatures?" Marsha asked, forcing herself to look into the dissecting pan. The animals seemed to be struggling against the pins that held them on the bottom of the tray.
         "The techs will clean up in the morning," Victor said, missing her meaning. He turned on the light of the microscope.
         With one last look at the Aplasia, Marsha went over to Victor, who was already busily peering down and adjusting the focus on the two-man dissecting scope.
         She bent over and looked. The ganglia were in the shape of the letter H with the swollen crosspiece resembling a transparent bag of clear marbles. The arms of the H were undoubtedly transsected nerve fibers. Victor was moving a pointer, and he told Marsha to count the nerve cells or neurons as he indicated them.
         Marsha did as she was told.
         "Okay," Victor said. "Let's look at the other ganglion."
         The visual field rushed by, then stopped. There was another H like the first. "Count again," Victor said.
         "This one has more than twice as many neurons as the other."
         "Precisely!" Victor said, straightening up and getting to his feet. He began to pace. His face had an odd, excited sheen, and Marsha began to feel the beginnings of fear. "I got very interested in the number of nerve cells of normal Aplasia about twelve years ago. At that time I knew, like everyone else, that nerve cells differentiated and proliferated during early embryological development. Since these Aplasia were relatively less complicated than higher animals, I was able to isolate the protein which was responsible for the process which I called nerve growth factor, or NGF. You follow me?" Victor stopped his pacing to look directly at Marsha.
         "Yes," Marsha said, watching her husband. He seemed to be changing in front of her eyes. He'd developed a disturbing messianic appearance. She suddenly felt queasy, with the awful thought that she knew where this seemingly irrelevant lecture was heading.
         Victor recommenced his pacing as his excitement grew. "I used genetic engineering to reproduce the protein and isolate the responsible gene. Then, for the brilliant part . . ." He stopped again in front of Marsha. His eyes sparkled. "I took a fertilized Aplasia egg or zygote and after causing a point mutation in its DNA, I inserted the new NGF gene along with a promoter. The result?"
         "More ganglionic neurons," Marsha answered.
         "Exactly," Victor said excitedly. "And, equally as important, the ability to pass the trait on to its offspring. Now, come back into the main room." He gave Marsha a hand, and pulled her to her feet.
         Dumbly she followed him to a light box, where he displayed some large transparencies of microscopic sections of rat brains. Even without counting, Marsha was able to appreciate that there were many more nerve cells in one photograph than the other. Still speechless, she let him herd her into the animal room itself. Just inside the door he slipped on a pair of heavy leather gloves.
         Marsha tried not to breathe. It smelled like a badly run zoo. There were hundreds of cages